FastQCFastQC Report
Wed 8 Jan 2025
SRR2125766.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2125766.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15226818
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC8118445.331672053872319RNA PCR Primer, Index 1 (100% over 50bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC3338062.1922242716764595RNA PCR Primer, Index 1 (100% over 36bp)
GTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGG3090952.029938231349452No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA2229911.4644622402395562RNA PCR Primer, Index 1 (100% over 37bp)
TTGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACT2210141.45147856893016No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT1652791.085446742714072RNA PCR Primer, Index 1 (100% over 35bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA1545231.0148082153474218RNA PCR Primer, Index 1 (100% over 34bp)
AATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGGAA1392140.9142684965434013RNA PCR Primer, Index 1 (100% over 23bp)
TCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAAG1370820.9002668843877952No Hit
GACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAA1093920.7184166777326688No Hit
ACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAG845480.5552571784860106No Hit
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC812690.5337228040684534RNA PCR Primer, Index 1 (100% over 33bp)
TGACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCA783260.5143950627110667No Hit
TAGTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACTTG652040.42821816087904907No Hit
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACG642440.42191349499284747RNA PCR Primer, Index 1 (100% over 39bp)
GCTGACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGC575140.37771516018645523No Hit
AGTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACTTGG571010.375002840383329No Hit
TGCTGGCGACAATAGCAAGAGTGAACCACCTGATCCCTTCCCGAACTCAG570510.3746744723684226No Hit
ATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGGAAC527960.34673035429989374RNA PCR Primer, Index 1 (100% over 24bp)
TTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAATTGCTC514270.3377396380517584No Hit
GCTGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTC511430.3358745077270905No Hit
CGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAAGG454880.29873608524118433Illumina Small RNA Adapter 2 (100% over 21bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCT451160.2962930272102812RNA PCR Primer, Index 1 (100% over 43bp)
CTGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCT435090.2857392792111917No Hit
GATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAA431980.28369683015847436No Hit
TGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCTG395240.25956834842315707No Hit
TGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAAT369340.2425588852510091No Hit
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATG368890.24226335403759341RNA PCR Primer, Index 1 (100% over 49bp)
GTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACTTGGA357850.23501298826846162No Hit
ACGTTGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAAT351790.23103316792779685No Hit
GTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAA351020.2305274811848411No Hit
GAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCT347010.22789396970529235No Hit
TTGTAATCAGTAGGTCCACAGTTCGAATCCGTGTGCCGGCACCATGGAAT345980.22721753159458527No Hit
ATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGGGTGCCAAG314230.2063661626480332No Hit
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGT313040.20558464677255614RNA PCR Primer, Index 1 (100% over 46bp)
TGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTA293100.19248933033809165No Hit
ACGACGTTGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACC291520.19145168741098764No Hit
TAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAATT283170.1859679415620519No Hit
ATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAAT278440.182861580141038No Hit
GTGGGGTTACCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGG274830.18049076307341427No Hit
ATAGTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACTT270420.17759455718194045No Hit
CAGTAATGCATTAAGCTAACCAGTACTAATTGCCCGTGCGGCTTGACCTG263820.17326009938517686No Hit
AGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTG259530.17044270181728055No Hit
AGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAATTG257220.16892564158841328No Hit
GGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCC251980.16548434479219493No Hit
AGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGGGTGC245490.16122212795871074No Hit
CCCGAACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGTTAT245260.16107107867185383No Hit
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC244400.16050628568621494RNA PCR Primer, Index 1 (98% over 50bp)
AACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTC243150.1596853656489491No Hit
CAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGGA242800.1594555080385147RNA PCR Primer, Index 1 (100% over 22bp)
AAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGT232500.15269112693144424No Hit
TTCCCGAACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGTT227470.14938774470148655No Hit
CAGTAATGCATTAAGCTAACCAGTACTAATTGCCCGTGCGGCTTGACTGG220920.1450861237062136No Hit
CCGAACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGTTATC220140.14457386960295973No Hit
CCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGG218830.14371354540390513Illumina Small RNA Adapter 2 (100% over 21bp)
AGCTGACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTG215610.14159885538790837No Hit
TATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGGGTGCCAA215070.14124421793180952No Hit
CGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTC210050.13794740306214995No Hit
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCAC207390.13620048522284828RNA PCR Primer, Index 1 (100% over 38bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA206660.13572106792108504RNA PCR Primer, Index 1 (100% over 32bp)
CTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAA206230.13543867142826557No Hit
CTTCCCGAACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGT201970.13264097594126362No Hit
GTCCCTATCGTCTAGAGGCCTAGGACATCGCCCTTTCACGGCGGTAACCG200620.13175438230101655No Hit
CGCTGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCT191540.12579121915031755No Hit
AACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGTTATCCAT190480.12509507895871613No Hit
TTAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTT189780.12463536373784727No Hit
GCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATT184840.12139108775057271No Hit
GACGTTGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAA183170.12029433858078556No Hit
AATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG170510.11198006044335725RNA PCR Primer, Index 1 (100% over 29bp)
CACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCAC169640.11140870009742022No Hit
TAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTA166010.10902474830920025No Hit
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG164510.10803964426448127RNA PCR Primer, Index 1 (100% over 45bp)
TCCCGAACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGTTA163440.10733693671258172No Hit
ACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACC163440.10733693671258172No Hit
ACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCG162080.10644377571203648No Hit
GTTATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGGGTGCC161350.10596435841027324No Hit
GAGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCG161150.10583301120431071No Hit
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGAT160920.10568196191745378RNA PCR Primer, Index 1 (100% over 41bp)
GTTGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATAC159500.10474939675511981No Hit
CTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGG158520.10410579544590341No Hit
AGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGC157210.10324547124684882No Hit
TAGAGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAG154080.10118988747353519No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)