FastQCFastQC Report
Wed 8 Jan 2025
SRR2125767.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2125767.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39998662
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATTGAGGATTGTTTAAAATTGAATAAAAAGGCTTTTTGGAATTCTCGG2895030.7237817104982162No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC2178990.5447657224134148RNA PCR Primer, Index 2 (100% over 50bp)
TTATTGAGGATTGTTTAAAATTGAATTGGAATTCTCGGGTGCCAAGGAAC1511710.3779401420977532RNA PCR Primer, Index 1 (100% over 24bp)
ATCCTTCTCTCCGTTTCTCCTTTTCTCTCTTTCTCCCTTTGGAATTCTCG1159050.28977219287985184No Hit
ATCCTTCTCTCCGTTTCTCCTTTTCTCTCTTTCTCCCTTTTGGAATTCTC1066180.2665539162284979No Hit
AATATCGAGTAACATTTGGAGGACTGGAATTCTCGGGTGCCAAGGAACTC865290.21632973622967688RNA PCR Primer, Index 1 (100% over 26bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA786980.19675158134039583RNA PCR Primer, Index 2 (100% over 36bp)
AATATCGAGTAACAGTTGGAGGACTGGAATTCTCGGGTGCCAAGGAACTC667870.1669730852497016RNA PCR Primer, Index 1 (100% over 26bp)
AGTGCATGGTTGTTTTCAATGGGGGATACTCAAACCCATCCAATATCGAG582850.14571737424616854No Hit
ATCGAGTAACATTTGGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAG555070.1387721419281475RNA PCR Primer, Index 1 (100% over 29bp)
TTATTGAGGATTGTTTAAAATTGAATAAAAAGGCTTTTTGGAAGGGGATT499130.12478667411424911No Hit
ATCGAGTAACAGTTGGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAG414210.10355596394699403RNA PCR Primer, Index 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)