FastQCFastQC Report
Wed 8 Jan 2025
SRR2125769.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2125769.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3988770
Total Bases203.4 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC1471783.6898091391581866RNA PCR Primer, Index 1 (100% over 50bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC434171.0884809101552608RNA PCR Primer, Index 1 (100% over 36bp)
AATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG267410.6704071681245096RNA PCR Primer, Index 1 (100% over 29bp)
TCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAAG262900.6591004244416199No Hit
GTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGG224090.5618022598445135No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA195270.48954940996848656RNA PCR Primer, Index 1 (100% over 37bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT191820.4809001271068525RNA PCR Primer, Index 1 (100% over 35bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA158510.3973906743181482RNA PCR Primer, Index 1 (100% over 34bp)
TGTAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC155720.39039603687352237RNA PCR Primer, Index 1 (98% over 50bp)
AATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGGAA153840.3856828044735595RNA PCR Primer, Index 1 (100% over 23bp)
GACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAA136610.3424865309356017No Hit
TCGGGAGTTCGAATCTCTCTGAGCGCACCGTGGAATTCTCGGGTGCCAAG126770.31781727199111504No Hit
ACCTGCGGCTGGATCACCTCCTTTGGAATTCTCGGGTGCCAAGGAACTCC117780.2952789957806542RNA PCR Primer, Index 1 (100% over 27bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT110850.27790521890206754RNA PCR Primer, Index 1 (100% over 30bp)
TGCTGGCGACAATAGCAAGAGTGAACCACCTGATCCCTTCCCGAACTCAG104700.26248693206176343No Hit
TTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAAGGAACTCC97370.24411033977892932RNA PCR Primer, Index 1 (100% over 27bp)
ACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAG95570.23959767045981595No Hit
ATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGGAAC95130.23849457351514378RNA PCR Primer, Index 1 (100% over 24bp)
TTATTGAGGATTGTTTAAAATTGAATAAAAAGGCTTTTTGGAATTCTCGG93110.23343035572369425No Hit
CGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAAGG89920.22543290287482107Illumina Small RNA Adapter 2 (100% over 21bp)
CTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC89500.22437994670036127RNA PCR Primer, Index 1 (100% over 27bp)
ATCCTTCTCTCCGTTTCTCCTTTTCTCTCTTTCTCCCTTTGGAATTCTCG87370.21903995467274373No Hit
CGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAG82530.2069058882813499RNA PCR Primer, Index 1 (100% over 29bp)
TGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAAT77390.19401971033677048No Hit
CCTGCGGCTGGATCACCTCCTTTGGAATTCTCGGGTGCCAAGGAACTCCA76520.19183858683253235RNA PCR Primer, Index 1 (100% over 28bp)
ACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGGAACTC72490.1817352216347395RNA PCR Primer, Index 1 (100% over 26bp)
GAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCT72310.18128395470282818No Hit
TGACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCA69790.17496621765606943No Hit
TAGTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACTTG69050.17311100915821168No Hit
TTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAATTGCTC65220.1635090516625426No Hit
CTGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCT63250.15857018579662402No Hit
GTCAAGTAATTAAGTGCATGTGGTGGAATTCTCGGGTGCCAAGGAACTCC62910.1577177927030137RNA PCR Primer, Index 1 (100% over 27bp)
GTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAA60250.15104907026476833No Hit
AATACTAATTGCTCGTGAGGCTTGACGTGGAATTCTCGGGTGCCAAGGAA56520.1416978166201611RNA PCR Primer, Index 1 (100% over 23bp)
CACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCAC55910.14016852312868378No Hit
AACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTC55860.14004317120315285No Hit
AAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGT54050.13550543149893327No Hit
AGTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACTTGG53890.1351043053372343No Hit
AGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTG52720.13217107027981057No Hit
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCA51790.1298395244649353RNA PCR Primer, Index 1 (100% over 28bp)
ACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACC50540.1267057263266621No Hit
CAGTAATGCATTAAGCTAACCAGTACTAATTGCCCGTGCGGCTTGACTGG48760.12224319777776106No Hit
GCTGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTC48740.1221930570075487No Hit
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC48620.12189221238627446RNA PCR Primer, Index 1 (100% over 33bp)
TCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAAGGAACTCCA47120.11813165462034664RNA PCR Primer, Index 1 (100% over 28bp)
TTCGAATCTCTCTGAGCGCACCGTGGAATTCTCGGGTGCCAAGGAACTCC47020.11788095076928477RNA PCR Primer, Index 1 (100% over 27bp)
GACCAATACTAATTGCTCGTGAGGCTTGACGTGGAATTCTCGGGTGCCAA47010.11785588038417857No Hit
TGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGGGTGCCAAGGAACTCC45480.11402011146293219RNA PCR Primer, Index 1 (100% over 27bp)
TAGAGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAG45310.11359391491612703No Hit
TAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGGAACTCCA45190.1132930702948528RNA PCR Primer, Index 1 (100% over 28bp)
GTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAAGGAACTC44350.1111871579459332RNA PCR Primer, Index 1 (100% over 26bp)
ATAGAGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGA44270.11098659486508372No Hit
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACG44090.11053532793317239RNA PCR Primer, Index 1 (100% over 39bp)
TTGTAATCAGTAGGTCCACAGTTCGAATCCGTGTGCCGGCACCATGGAAT43820.10985842753530536No Hit
ACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCG43790.10978321637998682No Hit
TATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGGGTGCCAA43510.10908124559701363No Hit
CGGGAGTTCGAATCTCTCTGAGCGCACCGTGGAATTCTCGGGTGCCAAGG43440.10890575290127033Illumina Small RNA Adapter 2 (100% over 21bp)
AGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGC42600.10679984055235073No Hit
ATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGGGTGCCAAG42230.10587223630342185No Hit
CGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTC42170.10572181399278474No Hit
AGAGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGC41940.10514519513534247No Hit
GCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCA41660.10444322435236929No Hit
GAGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCG40900.10253787508429917No Hit
TTGTAATCAGTAGGTCCACAGTTCGAATCCGTGTGCCGGCACCGTGGAAT39970.10020632926942391No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)