FastQCFastQC Report
Wed 8 Jan 2025
SRR2125770.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2125770.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2841970
Total Bases144.9 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC2382308.382565614696848RNA PCR Primer, Index 6 (100% over 50bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC1004703.535223806021879RNA PCR Primer, Index 6 (100% over 36bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCA524061.8440025756781386RNA PCR Primer, Index 6 (100% over 37bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC325661.1458952768678066RNA PCR Primer, Index 6 (100% over 35bp)
TGTAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC263960.9287923517841498RNA PCR Primer, Index 6 (98% over 50bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG227400.8001491922856329RNA PCR Primer, Index 6 (100% over 34bp)
GTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGG163290.5745662339855805No Hit
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAAT152630.5370570414184527RNA PCR Primer, Index 6 (100% over 39bp)
TCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAAG151330.5324827496419737No Hit
GACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAA115260.4055637462745912No Hit
TCGGGAGTTCGAATCTCTCTGAGCGCACCGTGGAATTCTCGGGTGCCAAG99830.3512704215737675No Hit
AATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGGAA98530.3466961297972885RNA PCR Primer, Index 1 (100% over 23bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCT93720.32977125022431625RNA PCR Primer, Index 6 (100% over 43bp)
ACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAG79820.2808615150758101No Hit
TTGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACT77900.2741056379905488No Hit
ATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGGAAC70900.2494748361172004RNA PCR Primer, Index 1 (100% over 24bp)
CGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAAGG59080.20788396781106067Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATG58560.20605425110046902RNA PCR Primer, Index 6 (100% over 49bp)
TTGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC55120.19394997132270925RNA PCR Primer, Index 6 (98% over 50bp)
TGACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCA54820.19289436552813718No Hit
GACCAATACTAATTGCTCGTGAGGCTTGACGTGGAATTCTCGGGTGCCAA53160.1870533467981717No Hit
TGCTGGCGACAATAGCAAGAGTGAACCACCTGATCCCTTCCCGAACTCAG51170.18005116169417693No Hit
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGT47330.16653940752365437RNA PCR Primer, Index 6 (100% over 46bp)
ACGTTCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATAT47240.16622272578528274RNA PCR Primer, Index 6 (100% over 41bp)
ATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGGGTGCCAAG46890.16499118569161533No Hit
GTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAA46670.1642170747755958No Hit
AATACTAATTGCTCGTGAGGCTTGACGTGGAATTCTCGGGTGCCAAGGAA46080.16214105004627072RNA PCR Primer, Index 1 (100% over 23bp)
CTGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCT46060.16207067632663258No Hit
TAGTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACTTG43070.15154980524073092No Hit
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAA42220.14855892215611002RNA PCR Primer, Index 6 (100% over 38bp)
TATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGGGTGCCAA42030.1478903718195477No Hit
TTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAATTGCTC41870.1473273820624426No Hit
TGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCTG40060.1409585604351911No Hit
AGTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACTTGG38520.13553978402305444No Hit
CGTTCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATC37680.13258408779825262RNA PCR Primer, Index 6 (100% over 42bp)
CGGGAGTTCGAATCTCTCTGAGCGCACCGTGGAATTCTCGGGTGCCAAGG37380.13152848200368056Illumina Small RNA Adapter 2 (100% over 21bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC36320.1277986748628592RNA PCR Primer, Index 1 (100% over 33bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATAT34970.12304844878728487RNA PCR Primer, Index 6 (100% over 41bp)
TGGAATCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGCC34600.12174653497397932RNA PCR Primer, Index 6 (97% over 47bp)
ACCAATACTAATTGCTCGTGAGGCTTGACGTGGAATTCTCGGGTGCCAAG34340.12083167661868352No Hit
CAGTAATGCATTAAGCTAACCAGTACTAATTGCCCGTGCGGCTTGACTGG34030.11974088396429239No Hit
AATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG33460.11773523295460543RNA PCR Primer, Index 1 (100% over 29bp)
ATACTAATTGCTCGTGAGGCTTGACGTGGAATTCTCGGGTGCCAAGGAAC33340.1173129906367766RNA PCR Primer, Index 1 (100% over 24bp)
GCTGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTC32530.114462854991432No Hit
GCTGACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGC31270.11002931065422927No Hit
GAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCT30930.10883295742038093No Hit
AGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGGGTGC30700.10802365964454234No Hit
TGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAAT30190.1062291297937698No Hit
GTCGGGAGTTCGAATCTCTCTGAGCGCACCGTGGAATTCTCGGGTGCCAA29780.10478646854118798No Hit
CACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCAC29460.10366048902697776No Hit
GTCCCTATCGTCTAGAGGCCTAGGACATCGCCCTTTCACGGCGGTAACCG29260.10295675183059638No Hit
TCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATC28450.10010661618525178RNA PCR Primer, Index 6 (100% over 42bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)