FastQCFastQC Report
Wed 8 Jan 2025
SRR2125771.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2125771.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14683932
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG3096012.108433899040121TruSeq Adapter, Index 2 (100% over 49bp)
AAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCA544910.37109270187304055Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGTCCCGAGGCGCTGAACCTTGATGCTTGGAATTTCATGCTCAGGGACT513140.349456807618014No Hit
ACAGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACT470180.32020033871036724Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT459020.3126001945527942Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATCAGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAAC390310.2658075507296002Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CATCCCCTTTAATAGCGAGTGGTCTTTGTGTGCATCTCCGCAAACCACTC382690.2606182049876014No Hit
GGCATCGCCGTCTCGACTTTTACTAGGCGGCGCAGCCGATTAGCGTTTAA364720.24838033845430504No Hit
GTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT348350.23723209832352807Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GGTAAGACGGAGGTCGGGGGTTCGATCCCCCCAGTGGCCTCCAAGATCGG301510.2053332853897716No Hit
GCGCGGTTAGTCTAGTGGAAAGTCACTCGCACAGATCGGAAGAGCACACG300450.20461140789810248No Hit
ATCTCGAATCGCCACTTCTCTTATCTGGTGGGCTCTGCCCGCCCTTGTCG300270.20448882492781906No Hit
TACGTCCCGAGGCGCTGAACCTTGATGCTTGGAATTTCATGCTCAGGGAC280820.19124305397219218No Hit
CAGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTC249540.16994085780293725Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCAGAGTTCTACAGTCCGACGATAGATCGGAAGAGCACACGTCTGAACT245670.1673053239418434Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCC240780.16397515324914336Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTAT229990.15662698519715293TruSeq Adapter, Index 2 (100% over 48bp)
ATATATATAACTAACGAATTACTATTTTGATATCTTGGACGTCGAATTAA226460.15422299694659442No Hit
ATCTGGTGGGCTCTGCCCGCCCTTGTCGGCGCCCAGTACCTTCATTTTCA201680.13734740803757467No Hit
ATCTCGAATCGCCACTTCTCTTATCTGGTGGGCTCTGCCCGCCCAGATCG195080.132852699127182No Hit
TCTCGAATCGCCACTTCTCTTATCTGGTGGGCTCTGCCCGCCCTTGTCGG188430.12832393939171063No Hit
ATATATATAACTAACGAATTACTATTTTGATATCTTGGACGTCGAAAGAT185770.12651243549752206No Hit
AGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAG184810.1258586596560104Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTGAACCTTGATGCTTGGAATTTCATGCTCAGGGACTAAGATCGGAAGAG178430.12151377437596415No Hit
TTCTCGGCTGAACGCCTCTAAGCCAGAAACCAGTCCCAAGACCGGGTGCC176410.12013812104278336No Hit
TGAACCTTGATGCTTGGAATTTCATGCTCAGGGACTAAGATCGGAAGAGC175280.11936857239600403No Hit
ATCTCGAATCGCCACTTCTCTTATCTGGTGGGCTCAGATCGGAAGAGCAC155720.10604788962520392No Hit
ATATAAAACCACAAAGGTAGAAGATCGTAATAGAGATAGAATAATATTTT147170.10022519853674071No Hit
AAATATAAAACATCAAGATAAATGGATTGTGATAGAGAAAGTTAAATTTT147160.10021838837172496No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)