FastQCFastQC Report
Wed 8 Jan 2025
SRR2125761.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2125761.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33681553
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length20-75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGGCGCTGGAACCCGGGGTGTTGTACTCTC1691860.5023105674491909No Hit
GGTAAGACGGAGGTCGGGGGTTCGATCCCCCCAGTGGCCTCCA1258700.37370604615529457No Hit
AACGTGTCGCGATGGATGACTTGGCTTCCTATTTCGTTGAAGAACGCAGC1191600.3537841619120116No Hit
ATGGCGCTGGAACCCGGGGTGTTGTACTCTC1093320.3246049848117158No Hit
GGCGCTGGAACCCGGGGTGTTGTACTCTC848290.25185596400498517No Hit
CTAAGCCAGAAACCAGTCCCAAGACCGGGTGCCCGT832570.24718872078137252No Hit
ATACATTAATACAAAATCAGACTATGTGAGTTAGATGACGTGAATTATTT791730.23506338914954425No Hit
ATACAATCATACACAGTAGTACATATGTAGTGATAGACGTGATTAATTTT688670.20446503758303544No Hit
AAGACGCTAAGGGTTGGATTGTTCACCCACT559050.16598106387790373No Hit
TTGGGCGTTTGAGGGTTGGTGACCCGTGGGCGCCCAGCCGTGCGGAACT499630.14833935953012617No Hit
ATATATACAATAACAGTGGTAAGGTAA495960.14724974231443544No Hit
ATATATAACAACATAAAATACTATGTGATGTAGGATCTGTGAATTAATTT481440.14293877719949552No Hit
ATATATAAAACTACGAGTAGATTCTATGATTGATGAACGTGTAAATTTTT472420.14026075341597224No Hit
TGGGCGTTTGAGGGTTGGTGACCCGTGGGCGCCCAGCCGTGCGGAACT471570.1400083897556624No Hit
CCCCGGTGGAGGCCTGTAGCGATGCTGACGT461740.13708987824878502No Hit
GGCAAGTGCTCCGACGCTATCGCACGGTGGTTCTCGGCTGAACG456950.13566773479833308No Hit
GGGTCGGTGTGTTGTGTGTGGGTT445110.13215245745942888No Hit
GAAGACGCTAAGGGTTGGATTGTTCACCCACT436390.1295635032030738No Hit
ATATATAACGACACAATAGAGAAAGATGCTCTGAGAGATGTAATATTTTT424850.1261372953913378No Hit
GTCGGTGTGTTGTGTGTGGGTT417780.12403822353440769No Hit
AGAGTTCTACAGTCCGACGATC415140.1232544116953277Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGGCGCTGGAACCCGGGGTGTTGTACTCTC405020.1202497996455211No Hit
ATATATTAAAACATATATTAGTATTTCGGTACATAGAGCTCACGTTTTTT399650.11865545510921066No Hit
GGTCGGTGTGTTGTGTGTGGGTT398320.11826058020543175No Hit
CGGTGTGTTGTGTGTGGGTT389150.11553802165832436No Hit
ATACATAAATAGATAAGATAGTATATGATGAGACTGTGATGATTGTAATT376750.11185648120203959No Hit
CCTCTAAGCCAGAAACCAGTCCCAAGACCGGGTGCCCGT363430.1079017941957724No Hit
AGTTCTACAGTCCGACGATC352120.104543873021532No Hit
ATGACCTCGAACTGTGGCACCATTTTTGTGGCCGTTCTTATAGGGCTGGT348210.10338300018410672No Hit
AGACGCTAAGGGTTGGATTGTTCACCCACT346120.1027624824781684No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)