FastQCFastQC Report
Wed 8 Jan 2025
SRR2125766.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2125766.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12358149
Total Bases580.2 Mbp
Sequences flagged as poor quality0
Sequence length20-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTC3110252.5167603983412077No Hit
TTGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACT2206021.7850731529454777No Hit
AATACTAATTGCTCGTGAGGCTTGACT1427571.1551649037408436No Hit
TCGGGAGTTCGAATCTCTCTGAGCGCACCA1395121.128906926110051No Hit
GACCAATACTAATTGCTCGTGAGGCTTGACT1109410.897715345558627No Hit
ACCAATACTAATTGCTCGTGAGGCTTGACT875080.7080995705748491No Hit
TGACCAATACTAATTGCTCGTGAGGCTTGACT794790.6431302940270424No Hit
TAGTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACT650870.5266727242081318No Hit
GCTGACCAATACTAATTGCTCGTGAGGCTTGACT580770.46994901906426284No Hit
TGCTGGCGACAATAGCAAGAGTGAACCACCTGATCCCTTCCCGAACTCAG568570.4600769904942884No Hit
AGTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACT568040.45964812367936325No Hit
ATACTAATTGCTCGTGAGGCTTGACT547320.44288185876380026No Hit
TTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAATTGCTC513470.4154910253954698No Hit
GCTGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTC501890.40612069008069085No Hit
CGGGAGTTCGAATCTCTCTGAGCGCACCA470390.38063143598608495No Hit
CTGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTC440720.3566229861769752No Hit
GATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAA432440.3499229536721074No Hit
TGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTC396720.3210189487114939No Hit
TGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCA369020.2986045887616341No Hit
GTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACT360490.2917022605893488No Hit
GTCGGGAGTTCGAATCTCTCTGAGCGCACCA357940.2896388447816902No Hit
ACGTTGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAAT350860.28390983148042637No Hit
GAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCA348990.28239665988814344No Hit
TTGTAATCAGTAGGTCCACAGTTCGAATCCGTGTGCCGGCACCA345370.2794674186239379No Hit
ATCCATGTGAGAGTAAGTCATCGCCAGCTC321130.2598528307111364No Hit
TGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTA296590.23999548799743392No Hit
ACGACGTTGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACC290970.23544788139388834No Hit
TAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAATT284520.23022865317451668No Hit
ATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAAT277420.2244834562198595No Hit
GTGGGGTTACCCATGTGAGAGTAAGTCATCGCCAGCTC276300.22357717162982904No Hit
ATAGTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACT268900.2175892198742708No Hit
AGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCA263450.21317917432456918No Hit
CAGTAATGCATTAAGCTAACCAGTACTAATTGCCCGTGCGGCTTGACC262460.21237808348159581No Hit
GGGTCGGGAGTTCGAATCTCTCTGAGCGCACCA255690.2068999168079297No Hit
AGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAATTG255180.206487233646398No Hit
CAATACTAATTGCTCGTGAGGCTTGACT250180.2024413202980479No Hit
AGTTATCCATGTGAGAGTAAGTCATCGCCAGCTC248740.20127609725372303No Hit
CCCGAACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGTTAT245000.19824975406915712No Hit
AACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCA243990.19743247957279042No Hit
AAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCA236120.19106421196248727No Hit
TTCCCGAACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGTT227150.1838058434155471No Hit
CCAATACTAATTGCTCGTGAGGCTTGACT226560.18332842564044177No Hit
CAGTAATGCATTAAGCTAACCAGTACTAATTGCCCGTGCGGCTTGAC220110.1781091974210701No Hit
TATCCATGTGAGAGTAAGTCATCGCCAGCTC220020.1780363709807998No Hit
CCGAACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGTTATC219780.177842167140079No Hit
AGCTGACCAATACTAATTGCTCGTGAGGCTTGACT218740.17700061716362214No Hit
CGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCA211480.17112595098181774No Hit
CTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCA205920.1666268953384524No Hit
CTTCCCGAACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGT201470.16302603245842076No Hit
CGCTGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCT191760.15516886873592478No Hit
AACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGTTATCCAT191140.15466717548072936No Hit
AATTCCACCACGTTCCCGTGG191040.15458625721376235No Hit
GCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCA185540.1501357525305772No Hit
GACGTTGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAA183300.14832318335051634No Hit
TTAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTT174750.14140467152483757No Hit
CACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCAC169450.1371160033755864No Hit
TAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTA165930.1342676803783479No Hit
GTTATCCATGTGAGAGTAAGTCATCGCCAGCTC165860.13421103759147102No Hit
ACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCA165270.13373361981636572No Hit
ACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACC163680.13244701937159037No Hit
TCCCGAACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGTTA163550.13234182562453325No Hit
GAGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCG160800.13011657328294068No Hit
CTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCA160110.12955823724086835No Hit
GTTGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATAC159850.12934784974675415No Hit
AGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGC157340.12731680124588238No Hit
TAGAGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAG154110.12470314122284819No Hit
CATGTGAGAGTAAGTCATCGCCAGCTC153130.12391014220657155No Hit
GGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCA149920.12131266583693076No Hit
GATAGAGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTG147820.1196133822306237No Hit
AGAGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGC145570.11779272122386614No Hit
CTGACCAATACTAATTGCTCGTGAGGCTTGACT144410.1168540693270489No Hit
ATGTGAGAGTAAGTCATCGCCAGCTC143530.11614198857773927No Hit
CATAGTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACT142920.11564838714924056No Hit
ACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGTTATCCATG141820.11475828621260352No Hit
GTCCCTATCGTCTAGAGGCCTAGGACATCGCCCTTTCACGGCGGTAACCG141140.1142080419972279No Hit
CTGGATAGAGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCT136750.11065573007737649No Hit
CCTTCCCGAACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAG136310.11029968970272166No Hit
ATAGAGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGA135620.10974135366064934No Hit
GAATTCCACCACGTTCCCGTGG133630.10813108014800599No Hit
GATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAATTGCTCGTG131980.10679592874305044No Hit
TGCTGGCGACAATAGCAAGAGTGAACCACCTGATCCCTTCCCGA130810.1058491850195365No Hit
GGATAGAGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCT130420.1055336037783652No Hit
GCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCA129580.10485389033584236No Hit
GAACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGTTATCCA129400.10470823745530176No Hit
GGGAGTTCGAATCTCTCTGAGCGCACCA125750.10175472071100615No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)