FastQCFastQC Report
Wed 8 Jan 2025
SRR2125769.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2125769.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3541626
Total Bases125.8 Mbp
Sequences flagged as poor quality0
Sequence length20-51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTCCACCACGTTCCCGTGG290490.8202164768386047No Hit
TCGGGAGTTCGAATCTCTCTGAGCGCACCA271910.7677546979833557No Hit
GTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTC224510.6339178671039799No Hit
AATACTAATTGCTCGTGAGGCTTGACT163930.46286649126700563No Hit
GACCAATACTAATTGCTCGTGAGGCTTGACT138690.3915997906046545No Hit
TCGGGAGTTCGAATCTCTCTGAGCGCACCG129770.3664136190552023No Hit
ACCTGCGGCTGGATCACCTCCTT126060.3559382046551499No Hit
ATTCCACCACGTTCCCGTGG122230.34512396283514973No Hit
ACCAATACTAATTGCTCGTGAGGCTTGACT105520.2979422446074204No Hit
TTCGAATCTCTCTGAGCGCACCA102690.28995156462031846No Hit
TGCTGGCGACAATAGCAAGAGTGAACCACCTGATCCCTTCCCGAACTCAG102270.28876566865050124No Hit
ATACTAATTGCTCGTGAGGCTTGACT101940.2878338932456448No Hit
CTAATTGCTCGTGAGGCTTGACT96590.2727278374396393No Hit
CGGGAGTTCGAATCTCTCTGAGCGCACCA96440.27230430316470455No Hit
TTATTGAGGATTGTTTAAAATTGAATAAAAAGGCTTTT93330.26352302586439114No Hit
CGAATCTCTCTGAGCGCACCA89560.2528781977543648No Hit
ATCCTTCTCTCCGTTTCTCCTTTTCTCTCTTTCTCCCTT86410.24398397798073543No Hit
CCTGCGGCTGGATCACCTCCTT82990.23432739651222345No Hit
ACTAATTGCTCGTGAGGCTTGACT77530.21891074890459916No Hit
TGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCA76500.216002480216714No Hit
GAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCA72910.20586589323660942No Hit
TGACCAATACTAATTGCTCGTGAGGCTTGACT71310.20134819430397224No Hit
TAGTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACT67120.18951747022412868No Hit
GTCAAGTAATTAAGTGCATGTGG64980.1834750479017265No Hit
CTGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCT64160.18115972719874995No Hit
TTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAATTGCTC64050.18084913539713116No Hit
GTCGGGAGTTCGAATCTCTCTGAGCGCACCA62710.17706556254104752No Hit
GAATTCCACCACGTTCCCGTGG60560.17099490460031636No Hit
AATACTAATTGCTCGTGAGGCTTGACG59520.16805840029410218No Hit
AACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCA56210.1587124106272091No Hit
AAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCA55440.15653826801587745No Hit
CACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCAC54670.1543641254045458No Hit
AGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCA54390.15357352809133432No Hit
AGTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACT52840.14919700725034207No Hit
TCGAATCTCTCTGAGCGCACCA52320.14772875509723501No Hit
TAATTGCTCGTGAGGCTTGACT49620.1401051381484098No Hit
TTCGAATCTCTCTGAGCGCACCG49230.1390039490335795No Hit
ACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACC48930.13815688048371003No Hit
TGAGAGTAAGTCATCGCCAGCTC48160.13598273787237838No Hit
GTTCGAATCTCTCTGAGCGCACCA47440.13394977335269168No Hit
GACCAATACTAATTGCTCGTGAGGCTTGACG47370.1337521240243888No Hit
CGGGAGTTCGAATCTCTCTGAGCGCACCG46350.13087209095483263No Hit
GCTGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTC46190.1304203210615689No Hit
TATCCATGTGAGAGTAAGTCATCGCCAGCTC45750.12917795385509367No Hit
ACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCA45630.1288391264351459No Hit
ATCCATGTGAGAGTAAGTCATCGCCAGCTC44800.12649557011384038No Hit
CAGTAATGCATTAAGCTAACCAGTACTAATTGCCCGTGCGGCTTGAC44580.12587438651060276No Hit
TAGAGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAG43850.12381318637258705No Hit
ATAGAGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGA43510.12285317534940167No Hit
CGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCA42750.12070726835639901No Hit
TTGTAATCAGTAGGTCCACAGTTCGAATCCGTGTGCCGGCACCA41880.11825076956177756No Hit
AGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGC41740.1178554709051718No Hit
AGTTCGAATCTCTCTGAGCGCACCA40760.11508838030893154No Hit
AGAGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGC40580.11458013917900987No Hit
CAATACTAATTGCTCGTGAGGCTTGACT40370.11398719119410125No Hit
GCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCA40200.11350718568250853No Hit
CGAATCTCTCTGAGCGCACCG39610.1118412842010986No Hit
GAGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCG39190.11065538823128135No Hit
CTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCA39060.11028832519300456No Hit
TTGTAATCAGTAGGTCCACAGTTCGAATCCGTGTGCCGGCACCG38950.10997773339138577No Hit
GATAGAGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTG38520.10876360180323953No Hit
CATGTGAGAGTAAGTCATCGCCAGCTC38430.1085094812382787No Hit
AGTTATCCATGTGAGAGTAAGTCATCGCCAGCTC38060.10746476336010635No Hit
TACTAATTGCTCGTGAGGCTTGACT37850.10687181537519773No Hit
CTAATTGCTCGTGAGGCTTGACG37100.10475414400052405No Hit
TGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCG36490.10303177128245614No Hit
TGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTC36320.10255176577086345No Hit
GAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCG36200.10221293835091566No Hit
TTGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACT36160.10209999587759973No Hit
ACCAATACTAATTGCTCGTGAGGCTTGACG35660.1006882149611506No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)