FastQCFastQC Report
Wed 8 Jan 2025
SRR2125771.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2125771.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13650890
Total Bases956.6 Mbp
Sequences flagged as poor quality0
Sequence length20-75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG2865142.09886681381214TruSeq Adapter, Index 2 (100% over 49bp)
AGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCC1052630.771107231836166Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACGTCCCGAGGCGCTGAACCTTGATGCTTGGAATTTCATGCTCAGGGACT494840.36249651121648474No Hit
CATCCCCTTTAATAGCGAGTGGTCTTTGTGTGCATCTCCGCAAACCACTC357130.26161664184533023No Hit
TCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA341260.24999102622612884Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGCATCGCCGTCTCGACTTTTACTAGGCGGCGCAGCCGATTAGCGTTTAA325190.23821890001311272No Hit
AGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAG321910.2358161262745506Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT301940.2211870434821466Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAGAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTC298600.21874031656544005Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTC290690.2129458225800662Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATCTCGAATCGCCACTTCTCTTATCTGGTGGGCTCTGCCCGCCCTTGTCG278700.2041625124808712No Hit
TACGTCCCGAGGCGCTGAACCTTGATGCTTGGAATTTCATGCTCAGGGAC267770.19615570852889444No Hit
GGTAAGACGGAGGTCGGGGGTTCGATCCCCCCAGTGGCCTCCAAGATCGG265980.19484443871425233No Hit
GCGCGGTTAGTCTAGTGGAAAGTCACTCGCACAGATCGGAAGAGCACACG262760.19248561815383466No Hit
AAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCA255420.1871086793608329Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTCAGAGTTCTACAGTCCGACGATAGATCGGAAGAGCACACGTCTGAACT224050.16412849272098742Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCAGT221740.16243629536242693Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTAT213930.15671505667396043TruSeq Adapter, Index 2 (100% over 48bp)
ATATATATAACTAACGAATTACTATTTTGATATCTTGGACGTCGAATTAA212100.15537448474055537No Hit
CTGAACCTTGATGCTTGGAATTTCATGCTCAGGGACTAAGATCGGAAGAG205910.1508399818619885No Hit
TTCTCGGCTGAACGCCTCTAAGCCAGAAACCAGTCCCAAGACCGGGTGCC200820.14711128724940278No Hit
TCTCGAATCGCCACTTCTCTTATCTGGTGGGCTCTGCCCGCCCTTGTCGG194670.14260608648959885No Hit
ATCTGGTGGGCTCTGCCCGCCCTTGTCGGCGCCCAGTACCTTCATTTTCA191850.1405402871168107No Hit
TGAACCTTGATGCTTGGAATTTCATGCTCAGGGACTAAGATCGGAAGAGC185690.13602776082731602No Hit
ATCTCGAATCGCCACTTCTCTTATCTGGTGGGCTCTGCCCGCCCAGATCG182040.1333539424901966No Hit
ATATATATAACTAACGAATTACTATTTTGATATCTTGGACGTCGAAAGAT173290.12694410401080078No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC172100.12607236597760293TruSeq Adapter, Index 2 (100% over 50bp)
GAGTTCTACAGTCCGACGATCAGATCGGAAGAGCACACGTCTGAACTCCA155200.11369222080025551Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ATCTCGAATCGCCACTTCTCTTATCTGGTGGGCTCAGATCGGAAGAGCAC145430.10653517829240437No Hit
TCAGAGTTCTACAGTCCGACGATAGATCGGAAGAGCACACGTCTGAACTC142710.10454263421652361Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATATAAAACCACAAAGGTAGAAGATCGTAATAGAGATAGAATAATATTTT139770.1023889284874466No Hit
AAATATAAAACATCAAGATAAATGGATTGTGATAGAGAAAGTTAAATTTT139550.10222776683425037No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)