FastQCFastQC Report
Wed 8 Jan 2025
SRR2125761.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2125761.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33655244
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length20-75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGGCGCTGGAACCCGGGGTGTTGTACTCTC1691870.502706205309342No Hit
GGTAAGACGGAGGTCGGGGGTTCGATCCCCCCAGTGGCCTCCA1258700.37399817989731404No Hit
AACGTGTCGCGATGGATGACTTGGCTTCCTATTTCGTTGAAGAACGCAGC1191600.3540607223052669No Hit
ATGGCGCTGGAACCCGGGGTGTTGTACTCTC1093320.32485873523900166No Hit
GGCGCTGGAACCCGGGGTGTTGTACTCTC848290.2520528450187436No Hit
CTAAGCCAGAAACCAGTCCCAAGACCGGGTGCCCGT832570.24738195331461568No Hit
ATACATTAATACAAAATCAGACTATGTGAGTTAGATGACGTGAATTATTT791730.23524714306038014No Hit
ATACAATCATACACAGTAGTACATATGTAGTGATAGACGTGATTAATTTT688670.20462487212988262No Hit
AAGACGCTAAGGGTTGGATTGTTCACCCACT559050.16611081470691463No Hit
TTGGGCGTTTGAGGGTTGGTGACCCGTGGGCGCCCAGCCGTGCGGAACT499630.14845531947413604No Hit
ATATATACAATAACAGTGGTAAGGTAA496490.14752232965537257No Hit
ATATATAACAACATAAAATACTATGTGATGTAGGATCTGTGAATTAATTT481440.14305051539664965No Hit
ATATATAAAACTACGAGTAGATTCTATGATTGATGAACGTGTAAATTTTT472420.14037039814657115No Hit
TGGGCGTTTGAGGGTTGGTGACCCGTGGGCGCCCAGCCGTGCGGAACT471570.14011783720837084No Hit
CCCCGGTGGAGGCCTGTAGCGATGCTGACGT461750.13720001554586858No Hit
GGCAAGTGCTCCGACGCTATCGCACGGTGGTTCTCGGCTGAACG456950.1357737890713257No Hit
GGGTCGGTGTGTTGTGTGTGGGTT445110.1322557637674533No Hit
GAAGACGCTAAGGGTTGGATTGTTCACCCACT436390.12966478567203377No Hit
ATATATAACGACACAATAGAGAAAGATGCTCTGAGAGATGTAATATTTTT424850.12623589952282027No Hit
GTCGGTGTGTTGTGTGTGGGTT417790.12413815808318014No Hit
AGAGTTCTACAGTCCGACGATC415260.12338641787888985Illumina DpnII expression Sequencing Primer (100% over 22bp)
TGGCGCTGGAACCCGGGGTGTTGTACTCTC405020.12034380139986506No Hit
ATATATTAAAACATATATTAGTATTTCGGTACATAGAGCTCACGTTTTTT399650.11874821053147022No Hit
GGTCGGTGTGTTGTGTGTGGGTT398320.11835302694581565No Hit
CGGTGTGTTGTGTGTGGGTT389150.1156283401184077No Hit
ATACATAAATAGATAAGATAGTATATGATGAGACTGTGATGATTGTAATT376750.11194392172583863No Hit
CCTCTAAGCCAGAAACCAGTCCCAAGACCGGGTGCCCGT363430.10798614325898218No Hit
AGTTCTACAGTCCGACGATC352200.1046493675695829No Hit
ATGACCTCGAACTGTGGCACCATTTTTGTGGCCGTTCTTATAGGGCTGGT348210.10346381681261917No Hit
AGACGCTAAGGGTTGGATTGTTCACCCACT346130.10284578534031726No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)