FastQCFastQC Report
Wed 8 Jan 2025
SRR2125763.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2125763.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23463033
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length20-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC5555182.3676308173798333RNA PCR Primer, Index 12 (100% over 50bp)
TCTCGAATCGCCACTTCTCTTATCTGGTGGGCTCTGCCCGCCCTTGTCGG2049110.8733355146370035No Hit
ATCTCGAATCGCCACTTCTCTTATCTGGTGGGCTCTGCCCGCCCTTGTCG1853790.7900896699927926No Hit
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTT1843040.7855079946399087RNA PCR Primer, Index 12 (100% over 36bp)
ACGGAATCACGTAGACCCACTTGGGACCGAGTATTGCAATTATTGGTCGC1210790.5160415535365782No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT1155550.4924981352581314RNA PCR Primer, Index 12 (100% over 35bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTG1051230.4480367052290299RNA PCR Primer, Index 12 (100% over 37bp)
GACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAA983240.4190592068808836No Hit
TCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAAG911870.38864114456131904No Hit
CGGAATCACGTAGACCCACTTGGGACCGAGTATTGCAATTATTGGTCGCG822210.35042784110647585No Hit
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC822190.35041931705930773RNA PCR Primer, Index 2 (100% over 34bp)
CACGGAATCACGTAGACCCACTTGGGACCGAGTATTGCAATTATTGGTCG810670.3455094658904499No Hit
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC767000.3268972088987813RNA PCR Primer, Index 1 (100% over 33bp)
AACCACGGAATCACGTAGACCCACTTGGGACCGAGTATTGCAATTATTGG638320.27205348941886587No Hit
ACAATGTCGCTCCCGCTTGATCAAAAGAGCGGGGAAACCACGGAATCACG635750.2709581493577578No Hit
CCACGGAATCACGTAGACCCACTTGGGACCGAGTATTGCAATTATTGGTC632070.2693897246788171No Hit
ACCACGGAATCACGTAGACCCACTTGGGACCGAGTATTGCAATTATTGGT611750.2607292927559706No Hit
AGTGGACTGCCATGGCGTTGACGGGAGCGGGGGATTAGGGTTCGATTCCG532470.22693996978140038No Hit
CCCGCTTGATCAAAAGAGCGGGGAAACCACGGAATCACGTAGACCCACTT521860.22241796275869366No Hit
TGAAAAGGGGCAACAGAGAACCTGGGATGATATTCCAAAAAGAAATTGCA508400.21668127901452466No Hit
ATCACGTAGACCCACTTGGGACCGAGTATTGCAATTATTGGTCGCGCAAC502920.21434569009044996No Hit
AATGTCGCTCCCGCTTGATCAAAAGAGCGGGGAAACCACGGAATCACGTA485870.20707893987959697No Hit
AGACGTGTTGACTCAATTCATTCCGTGCGAAAGCCGAGCCTTGTTGCTCG465060.19820966880113067No Hit
CGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAAGG450200.1918763017551908Illumina Small RNA Adapter 2 (100% over 21bp)
ACGTAGACCCACTTGGGACCGAGTATTGCAATTATTGGTCGCGCAACGAG431490.1839020556293809No Hit
AATCACGTAGACCCACTTGGGACCGAGTATTGCAATTATTGGTCGCGCAA412100.17563799189985368No Hit
CACGTAGACCCACTTGGGACCGAGTATTGCAATTATTGGTCGCGCAACGA400770.17080911917909336No Hit
TCACGTAGACCCACTTGGGACCGAGTATTGCAATTATTGGTCGCGCAACG398180.16970525507081716No Hit
TTGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACT375660.16010717795947352No Hit
AGTGCATGGTTGTTTTCAATGGGGGATACTCAAACCCATCCAATATCGAG360160.15350104140415266No Hit
CACAATGTCGCTCCCGCTTGATCAAAAGAGCGGGGAAACCACGGAATCAC341010.1453392662406433No Hit
TGCTGGCGACAATAGCAAGAGTGAACCACCTGATCCCTTCCCGAACTCAG331800.1414139425197075No Hit
AAAAGAGCGGGGAAACCACGGAATCACGTAGACCCACTTGGGACCGAGTA331060.1410985527744857No Hit
CCGCTTGATCAAAAGAGCGGGGAAACCACGGAATCACGTAGACCCACTTG329980.1406382542274053No Hit
CAATGTCGCTCCCGCTTGATCAAAAGAGCGGGGAAACCACGGAATCACGT328200.13987961402943944No Hit
ACGCACTTGTCGTGGCGATGGGACGTCCAGCGAATGAATGAAATTAGAAC327980.1397858495105897No Hit
ATGTCGCTCCCGCTTGATCAAAAGAGCGGGGAAACCACGGAATCACGTAG327320.13950455595404054No Hit
GTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAA307270.1309591986679642No Hit
AAACCACGGAATCACGTAGACCCACTTGGGACCGAGTATTGCAATTATTG306490.13062676082840613No Hit
CTCCCGCTTGATCAAAAGAGCGGGGAAACCACGGAATCACGTAGACCCAC297630.12685060793291303No Hit
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATG293000.12487729101348492RNA PCR Primer, Index 12 (100% over 49bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTA290480.12380326107029727RNA PCR Primer, Index 12 (100% over 39bp)
GTAGACCCACTTGGGACCGAGTATTGCAATTATTGGTCGCGCAACGAGGA285310.12159979487732894No Hit
ACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAG280380.1194986172503785No Hit
GTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGG266600.11362554875151903No Hit
CTTGATCAAAAGAGCGGGGAAACCACGGAATCACGTAGACCCACTTGGGA264640.11279019212903975No Hit
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCG263960.11250037452532244RNA PCR Primer, Index 12 (100% over 45bp)
GAATCACGTAGACCCACTTGGGACCGAGTATTGCAATTATTGGTCGCGCA252500.10761609549796909No Hit
CGTAGACCCACTTGGGACCGAGTATTGCAATTATTGGTCGCGCAACGAGG252480.10760757145080094No Hit
TAGTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACTTG247380.10543393942292116No Hit
AGGGGCAACAGAGAACCTGGGATGATATTCCAAAAAGAAATTGCATGTGG243510.10378453629588298No Hit
ACGGAATCACGTAGACCCACTTGGGACCGAGTATTGCAATTTGGAATTCT237040.10102700703698451No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)