FastQCFastQC Report
Wed 8 Jan 2025
SRR2125764.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2125764.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16904135
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length20-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC7492294.4322232400533945RNA PCR Primer, Index 3 (100% over 50bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA2679291.584990891281926RNA PCR Primer, Index 3 (100% over 36bp)
GTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGG2276241.3465581054576292No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAG1680800.9943129299428808RNA PCR Primer, Index 3 (100% over 37bp)
TCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAAG1466170.8673439960104437No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT1438510.8509811356807078RNA PCR Primer, Index 3 (100% over 35bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT1422180.8413207774310841RNA PCR Primer, Index 3 (100% over 34bp)
TTGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACT1409790.8339912098430354No Hit
AATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGGAA1089240.6443630508156731RNA PCR Primer, Index 1 (100% over 23bp)
GACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAA955670.5653468811033514No Hit
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC718910.4252864757646576RNA PCR Primer, Index 1 (100% over 33bp)
ACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAG693550.41028422927289687No Hit
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC649300.3841072021727228RNA PCR Primer, Index 3 (100% over 39bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCT628450.3717729419458612RNA PCR Primer, Index 3 (100% over 43bp)
TGACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCA617870.36551411829117547No Hit
TGCTGGCGACAATAGCAAGAGTGAACCACCTGATCCCTTCCCGAACTCAG544840.3223116710792951No Hit
TAGTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACTTG527430.31201241589705714No Hit
AGTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACTTGG506530.29964857710850035No Hit
ATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGGAAC467590.27661279326034727RNA PCR Primer, Index 1 (100% over 24bp)
TTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAATTGCTC463750.27434115972216266No Hit
GCTGACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGC453120.2680527575057819No Hit
CTGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCT434280.2569075554590637No Hit
CGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAAGG428080.25323981380886984Illumina Small RNA Adapter 2 (100% over 21bp)
TGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCTG416140.24617645327607712No Hit
GCTGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTC374180.22135412430153925No Hit
TGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAAT362340.21434992089213672No Hit
GATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAA339990.20112830381442173No Hit
GAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCT339120.20061363684092678No Hit
TTGTAATCAGTAGGTCCACAGTTCGAATCCGTGTGCCGGCACCATGGAAT327820.1939288818978315No Hit
GTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAA326300.1930296936223001No Hit
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATG315680.18674720711825835RNA PCR Primer, Index 3 (100% over 49bp)
GTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACTTGGA294630.17429463264461625No Hit
ACGTTGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAAT293450.1735965785886116No Hit
ATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGGGTGCCAAG266210.15748217817711463No Hit
ATCTCGAATCGCCACTTCTCTTATCTGGTGGGCTCTGCCCGCCCTTGTCG259670.153613302307394No Hit
CCCGAACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGTTAT255760.15130025878283626No Hit
TAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAATT254160.1503537448085927No Hit
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGT253340.14986865639679284RNA PCR Primer, Index 3 (100% over 46bp)
TTCCCGAACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGTT251180.148590862531564No Hit
TGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTA248350.1469167159396207No Hit
ACGACGTTGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACC239900.14191793901314678No Hit
AGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTG238870.14130862064222746No Hit
ATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAAT238790.1412612949435153No Hit
CCGAACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGTTATC228920.13542248686490022No Hit
ATAGTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACTT228800.13535149831683194No Hit
AACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTC225610.1334643860806838No Hit
AACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGTTATCCAT225170.13320409473776684No Hit
AAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGT220050.13017525002018737No Hit
AGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGGGTGC218070.12900393897706094No Hit
CTTCCCGAACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGT215600.1275427580293224No Hit
AGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAATTG215400.12742444378254197No Hit
GGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCC214630.12696893393243724No Hit
CGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTC211090.12487477176442333No Hit
CAGTAATGCATTAAGCTAACCAGTACTAATTGCCCGTGCGGCTTGACCTG206660.12225411119823641No Hit
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGG205610.12163296140263906RNA PCR Primer, Index 3 (100% over 38bp)
CAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGGA204020.12069236314073449RNA PCR Primer, Index 1 (100% over 22bp)
CTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAA197220.11666967875019929No Hit
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCC195860.11586514187209224RNA PCR Primer, Index 3 (98% over 50bp)
CGCTGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCT194510.11506652070632421No Hit
TATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGGGTGCCAA194150.11485355506211943No Hit
CCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGG192820.11406676532102944Illumina Small RNA Adapter 2 (100% over 21bp)
TTAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTT189740.11224472592061055No Hit
GGTGGCTGTAGTTTAGTGGTGAGAATTCCACGTTGTGGAATTCTCGGGTG180030.1065005692394198No Hit
AGACGTGTTGACTCAATTCATTCCGTGCGAAAGCCGAGCCTTGTTGCTCG177940.10526418536056414No Hit
CAGTAATGCATTAAGCTAACCAGTACTAATTGCCCGTGCGGCTTGACTGG173430.10259619909566506No Hit
TCCCGAACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGTTA170380.10079190683226324No Hit
TAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTA170310.10075049684589008No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)