FastQCFastQC Report
Wed 8 Jan 2025
SRR2125765.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2125765.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8759946
Total Bases815.7 Mbp
Sequences flagged as poor quality0
Sequence length20-101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGC88504210.103281458584332RNA PCR Primer, Index 10 (100% over 50bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAG3223143.679406242915196RNA PCR Primer, Index 10 (100% over 36bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGC3028373.457064689668178RNA PCR Primer, Index 10 (100% over 37bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTA1722361.9661765038277632RNA PCR Primer, Index 10 (100% over 35bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT1634091.8654110424881614RNA PCR Primer, Index 3 (100% over 34bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1008381.1511258174422536RNA PCR Primer, Index 1 (100% over 33bp)
TCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAAG926361.057495103280317No Hit
GACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAA901071.0286250623006123No Hit
AATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGGAA589740.6732233280890088RNA PCR Primer, Index 1 (100% over 23bp)
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTT470370.5369553647933446RNA PCR Primer, Index 10 (100% over 39bp)
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATG468930.5353115190436105RNA PCR Primer, Index 10 (100% over 49bp)
GTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGG467190.5333252054293486No Hit
ATCTCGAATCGCCACTTCTCTTATCTGGTGGGCTCTGCCCGCCCTTGTCG431010.49202358096727994No Hit
TTGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACT411470.46971750739102736No Hit
CGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAAGG410440.46854170105614806Illumina Small RNA Adapter 2 (100% over 21bp)
TCTCGAATCGCCACTTCTCTTATCTGGTGGGCTCTGCCCGCCCTTGTCGG389380.4445004569662872No Hit
ACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAG323840.36968264416241836No Hit
TGCTGGCGACAATAGCAAGAGTGAACCACCTGATCCCTTCCCGAACTCAG273980.3127644850778761No Hit
GTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAA269600.30776445425576826No Hit
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGT264160.30155437031232846RNA PCR Primer, Index 10 (100% over 46bp)
ACGTTCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTAT248240.2833807423013795RNA PCR Primer, Index 10 (100% over 41bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCT238020.27171400371646126RNA PCR Primer, Index 10 (100% over 38bp)
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA229940.26249020256517563RNA PCR Primer, Index 1 (100% over 32bp)
TAGTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACTTG224070.2557892480159124No Hit
CTGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCT209100.23870010157596863No Hit
TGACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCA205340.23440783767388523No Hit
ATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGGAAC203930.23279823871060393RNA PCR Primer, Index 1 (100% over 24bp)
AGTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACTTGG199120.22730733728267274No Hit
TGGAATTCTCGGGTGCCAGGAACTCCAGTCACTAGCTTATCTCGTATGCC178690.20398527570832056RNA PCR Primer, Index 10 (97% over 35bp)
GAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCT177870.20304919687861087No Hit
TTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAATTGCTC174980.19975008978365846No Hit
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCG166350.18989843088073832RNA PCR Primer, Index 10 (100% over 45bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCT146020.1666905252612288RNA PCR Primer, Index 10 (100% over 43bp)
TGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAAT141910.1619987155171961No Hit
TGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCTG140350.16021788262165088No Hit
CGTTCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATC133490.1523867841194455RNA PCR Primer, Index 10 (100% over 42bp)
TTGTAATCAGTAGGTCCACAGTTCGAATCCGTGTGCCGGCACCATGGAAT133370.1522497969736343No Hit
GATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAA125270.14300316463138016No Hit
GCTGACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGC123470.14094835744421255No Hit
AACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTC119670.1366104311601921No Hit
ACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACC118830.13565152113951387No Hit
AGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTG116030.13245515440391983No Hit
TTCCCGAACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGTT113080.12908755373606184No Hit
CCCGAACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGTTAT112900.12888207301734508No Hit
CACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCAC112050.1279117474011826No Hit
GCTGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTC110320.12593684938240488No Hit
TAGAGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAG110230.12583410902304648No Hit
CGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTC107410.12261491109648393No Hit
GTCCCTATCGTCTAGAGGCCTAGGACATCGCCCTTTCACGGCGGTAACCG104380.11915598566475182No Hit
CGCTGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCT103740.11842538755375889No Hit
AAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGT102820.11737515276920656No Hit
GAGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCG102420.11691852894983598No Hit
ATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGGGTGCCAAG101440.11579980059237807No Hit
TTGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGC98360.11228379718322465RNA PCR Primer, Index 10 (98% over 50bp)
TATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGGGTGCCAA97500.11130205597157791No Hit
ACGTTGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAAT94350.10770614339403461No Hit
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTAT92540.10563992061138276RNA PCR Primer, Index 10 (100% over 41bp)
TGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTA91550.10450977665844058No Hit
TGGAATTCACGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGC90780.10363077580615224RNA PCR Primer, Index 10 (98% over 50bp)
AGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGC90110.10286593090870651No Hit
GATAGAGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTG90100.10285451531322225No Hit
CAGTAATGCATTAAGCTAACCAGTACTAATTGCCCGTGCGGCTTGACTGG90060.1028088529312852No Hit
ATAGAGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGA88340.10084537050799171No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)