FastQCFastQC Report
Wed 8 Jan 2025
SRR2125766.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2125766.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15223318
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length20-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC8080675.3080872382748625RNA PCR Primer, Index 1 (100% over 50bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC3332102.188813240319883RNA PCR Primer, Index 1 (100% over 36bp)
GTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGG3079732.023034663008419No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA2232941.4667893030941086RNA PCR Primer, Index 1 (100% over 37bp)
TTGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACT2206181.4492110064310553No Hit
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT1651961.0851510820440065RNA PCR Primer, Index 1 (100% over 35bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA1536191.009103271704631RNA PCR Primer, Index 1 (100% over 34bp)
AATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGGAA1390120.9131517846503633RNA PCR Primer, Index 1 (100% over 23bp)
TCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAAG1366060.8973470829421024No Hit
GACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAA1087120.7141150175014409No Hit
ACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAG854370.5612245635281349No Hit
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC810100.5321441751397429RNA PCR Primer, Index 1 (100% over 33bp)
TGACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCA778020.5110712395287282No Hit
TAGTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACTTG651330.42785022292774805No Hit
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACG642490.4220433416683538RNA PCR Primer, Index 1 (100% over 39bp)
GCTGACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGC571330.37529926130427016No Hit
AGTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACTTGG568910.37370959471516No Hit
TGCTGGCGACAATAGCAAGAGTGAACCACCTGATCCCTTCCCGAACTCAG568550.3734731153878543No Hit
ATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGGAAC528690.3472895987589565RNA PCR Primer, Index 1 (100% over 24bp)
TTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAATTGCTC513470.33729177831008983No Hit
GCTGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTC501900.3296915954852943No Hit
CGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAAGG459140.3016031064975454Illumina Small RNA Adapter 2 (100% over 21bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCT450090.29565827896388946RNA PCR Primer, Index 1 (100% over 43bp)
CTGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCT441530.29003532607017735No Hit
GATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAA432800.28430070238301536No Hit
TGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCTG397380.2610337641242205No Hit
TGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAAT368710.2422008132524066No Hit
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATG367410.2413468601260251RNA PCR Primer, Index 1 (100% over 49bp)
GTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACTTGGA360710.23694571709005882No Hit
ACGTTGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAAT350880.230488517680574No Hit
GTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAA350370.23015350530022433No Hit
GAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCT346550.2276441968827032No Hit
TTGTAATCAGTAGGTCCACAGTTCGAATCCGTGTGCCGGCACCATGGAAT345200.22675739940530704No Hit
ATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGGGTGCCAAG313830.20615085357870078No Hit
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGT311650.204718839874461RNA PCR Primer, Index 1 (100% over 46bp)
TGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTA296610.19483925908924715No Hit
ACGACGTTGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACC290980.19114098516499492No Hit
TAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAATT284590.1869434771053196No Hit
ATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAAT277480.18227301039103302No Hit
GTGGGGTTACCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGG273860.17989507937757065No Hit
ATAGTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACTT269360.17693908778625No Hit
CAGTAATGCATTAAGCTAACCAGTACTAATTGCCCGTGCGGCTTGACCTG262850.1726627532841395No Hit
AGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTG259300.1703308043620977No Hit
AGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAATTG255200.16763756757889442No Hit
GGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCC251130.1649640374063No Hit
CCCGAACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGTTAT245040.16096359545271274No Hit
AGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGGGTGC244690.16073368499561003No Hit
CAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGGA243990.1602738640814046RNA PCR Primer, Index 1 (100% over 22bp)
TGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCC243540.15997826492227255RNA PCR Primer, Index 1 (98% over 50bp)
AACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTC242420.15924255145954386No Hit
AAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGT232360.15263426803539148No Hit
TTCCCGAACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGTT227470.14942209050615643No Hit
CCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGG221250.14533625323993102Illumina Small RNA Adapter 2 (100% over 21bp)
CCGAACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGTTATC219850.14441661141152015No Hit
CAGTAATGCATTAAGCTAACCAGTACTAATTGCCCGTGCGGCTTGACTGG219840.14441004254131723No Hit
TATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGGGTGCCAA215170.14134238015654668No Hit
AGCTGACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTG215030.1412504159737056No Hit
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCAC212540.13961476729317485RNA PCR Primer, Index 1 (100% over 38bp)
CGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTC210520.13828785551218203No Hit
TCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA206060.13535813940167316RNA PCR Primer, Index 1 (100% over 32bp)
CTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAA205780.13517421103599098No Hit
CTTCCCGAACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGT201830.13257950730583173No Hit
CGCTGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCT191770.12597122388167942No Hit
AACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGTTATCCAT191160.12557052279930037No Hit
TTAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTT189290.1243421440713516No Hit
GCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATT184930.12147811666287205No Hit
GACGTTGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAA183370.12045337291121422No Hit
AATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG170880.11224885402774876RNA PCR Primer, Index 1 (100% over 29bp)
CACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCAC169450.11130950558872908No Hit
TAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCGGCAGGCTTA165930.10899726327729606No Hit
ACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACC163680.10751926748163573No Hit
ACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCG163610.1074732853902152No Hit
TCCCGAACTCAGAAGTGAAACCTCTTAGCGCTGATGGTAGTGTGGAGTTA163580.1074535787796064No Hit
GGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG163380.1073222013755477RNA PCR Primer, Index 1 (100% over 45bp)
GTTATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGGGTGCC162370.10665874548505129No Hit
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGAT161060.10579822348846685RNA PCR Primer, Index 1 (100% over 41bp)
GAGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCG160800.10562743286319054No Hit
GTTGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATAC159880.10502309680452053No Hit
CTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGG158340.10401149079326859No Hit
AGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGC157340.10335460377297512No Hit
TAGAGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAG154110.1012328586974272No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)