FastQCFastQC Report
Wed 8 Jan 2025
SRR2125767.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2125767.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39979049
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length20-51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATTGAGGATTGTTTAAAATTGAATAAAAAGGCTTTTTGGAATTCTCGG2818310.704946733475326No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC2112190.5283242230199122RNA PCR Primer, Index 2 (100% over 50bp)
TTATTGAGGATTGTTTAAAATTGAATTGGAATTCTCGGGTGCCAAGGAAC1476520.36932344238603576RNA PCR Primer, Index 1 (100% over 24bp)
ATCCTTCTCTCCGTTTCTCCTTTTCTCTCTTTCTCCCTTTGGAATTCTCG1125490.28151995311344197No Hit
ATCCTTCTCTCCGTTTCTCCTTTTCTCTCTTTCTCCCTTTTGGAATTCTC1030930.2578675645836398No Hit
AATATCGAGTAACATTTGGAGGACTGGAATTCTCGGGTGCCAAGGAACTC852440.21322168018553916RNA PCR Primer, Index 1 (100% over 26bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGA735130.18387881112429663RNA PCR Primer, Index 2 (100% over 36bp)
AATATCGAGTAACAGTTGGAGGACTGGAATTCTCGGGTGCCAAGGAACTC658310.16466374675395606RNA PCR Primer, Index 1 (100% over 26bp)
AGTGCATGGTTGTTTTCAATGGGGGATACTCAAACCCATCCAATATCGAG565660.14148910845778248No Hit
ATCGAGTAACATTTGGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAG537490.13444291783929127RNA PCR Primer, Index 1 (100% over 29bp)
TTATTGAGGATTGTTTAAAATTGAATAAAAAGGCTTTTTGGAAGGGGATT486820.12176877944245247No Hit
ATCGAGTAACAGTTGGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAG401020.10030753858102028RNA PCR Primer, Index 1 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)