FastQCFastQC Report
Wed 8 Jan 2025
SRR2125768.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2125768.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34837654
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length20-51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGC1866610.5358024395098476RNA PCR Primer, Index 10 (100% over 50bp)
AATATCGAGTAACATTTGGAGGACTGGAATTCTCGGGTGCCAAGGAACTC1072610.3078881258766735RNA PCR Primer, Index 1 (100% over 26bp)
TTATTGAGGATTGTTTAAAATTGAATTGGAATTCTCGGGTGCCAAGGAAC947770.27205333631248535RNA PCR Primer, Index 1 (100% over 24bp)
AATATCGAGTAACAGTTGGAGGACTGGAATTCTCGGGTGCCAAGGAACTC858020.24629098159135518RNA PCR Primer, Index 1 (100% over 26bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAG657670.18878136857321107RNA PCR Primer, Index 10 (100% over 36bp)
AGACGTGTTGACTCAATTCATTCCGTGCGAAAGCCGAGCCTTGTTGCTCG651070.18688686672185217No Hit
ACGGAATCACGTAGACCCACTTGGGACCGAGTTGGAATTCTCGGGTGCCA552550.1586071209042951No Hit
CCCGCTTGATCAAAAGAGCGGGGAAACCACGGAATCACGTAGACCCACTT518440.14881599088159037No Hit
AGTGCATGGTTGTTTTCAATGGGGGATACTCAAACCCATCCAATATCGAG500920.1437869496034377No Hit
ACGTAGACCCACTTGGGACCGAGTATTGCAATTATTGGTCGCGCAACGAG468440.1344637041288716No Hit
AAAAGAGCGGGGAAACCACGGAATCACGTAGACCCACTTGGGACCGAGTA441910.12684838077787902No Hit
CTTGATCAAAAGAGCGGGGAAACCACGGAATCACGTAGACCCACTTGGGA408020.11712040081688624No Hit
CACGTAGACCCACTTGGGACCGAGTATTGCAATTATTGGTCGCGCAACGA405600.11642575013805463No Hit
AAATGTGTTTCATCGTCTACTTATTACCTGGAATTCTCGGGTGCCAAGGA403510.115825824551791RNA PCR Primer, Index 1 (100% over 22bp)
AATGTCGCTCCCGCTTGATCAAAAGAGCGGGGAAACCACGGAATCACGTA403280.11575980403272851No Hit
ATGACCTCGAACTGTGGCACCATTTTTGTGGCCGTTCTTTGGAATTCTCG392720.1127286010705543No Hit
ACGGAATCACGTAGACCCACTTGGGACCGAGTATTGCAATTTGGAATTCT384630.11040640107396439No Hit
ATATGACCTCGAACTGTGGCACCATTTTTGTGGCCGTTCTTTGGAATTCT381860.10961128438786377No Hit
ATCGAGTAACATTTGGAGGACTGGAATTCTCGGGTGCCAAGGAACTCCAG375260.10771678253650491RNA PCR Primer, Index 1 (100% over 29bp)
TCACGTAGACCCACTTGGGACCGAGTATTGCAATTATTGGTCGCGCAACG357810.10270783445980604No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGC350230.10053202778809388RNA PCR Primer, Index 10 (100% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)