FastQCFastQC Report
Wed 8 Jan 2025
SRR2125769.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2125769.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3987378
Total Bases201.8 Mbp
Sequences flagged as poor quality0
Sequence length20-51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC1431433.5899029387231405RNA PCR Primer, Index 1 (100% over 50bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC422411.0593678352039861RNA PCR Primer, Index 1 (100% over 36bp)
TCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAAG255780.6414741717489538No Hit
AATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG254150.637386272382503RNA PCR Primer, Index 1 (100% over 29bp)
GTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGG218780.5486813640442416No Hit
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA193340.48488003896294757RNA PCR Primer, Index 1 (100% over 37bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT188880.47369474376394716RNA PCR Primer, Index 1 (100% over 35bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA153010.38373587856481123RNA PCR Primer, Index 1 (100% over 34bp)
AATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGGAA149950.3760616625762594RNA PCR Primer, Index 1 (100% over 23bp)
TGTAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC144240.3617414752250727RNA PCR Primer, Index 1 (98% over 50bp)
GACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAA130740.3278846399814615No Hit
TCGGGAGTTCGAATCTCTCTGAGCGCACCGTGGAATTCTCGGGTGCCAAG123190.3089498913822567No Hit
ACCTGCGGCTGGATCACCTCCTTTGGAATTCTCGGGTGCCAAGGAACTCC114520.28720627941469307RNA PCR Primer, Index 1 (100% over 27bp)
ATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGT107060.26849724305044564RNA PCR Primer, Index 1 (100% over 30bp)
TGCTGGCGACAATAGCAAGAGTGAACCACCTGATCCCTTCCCGAACTC97580.24472222096826537No Hit
ACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAG96850.24289144395138862No Hit
TTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAAGGAACTCC94950.23812640788006553RNA PCR Primer, Index 1 (100% over 27bp)
ATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGGAAC92990.23321089698543754RNA PCR Primer, Index 1 (100% over 24bp)
TTATTGAGGATTGTTTAAAATTGAATAAAAAGGCTTTTTGGAATTCTCGG90310.2264896882111503No Hit
CGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAAGG89710.2249849399781009Illumina Small RNA Adapter 2 (100% over 21bp)
CTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGGAACTCC88220.22124814853269492RNA PCR Primer, Index 1 (100% over 27bp)
ATCCTTCTCTCCGTTTCTCCTTTTCTCTCTTTCTCCCTTTGGAATTCTCG84120.21096570227352412No Hit
CGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAAGGAACTCCAG79370.19905311209521648RNA PCR Primer, Index 1 (100% over 29bp)
TGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAAT76040.19070175940179235No Hit
CCTGCGGCTGGATCACCTCCTTTGGAATTCTCGGGTGCCAAGGAACTCCA73740.1849335578417697RNA PCR Primer, Index 1 (100% over 28bp)
GAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCT70990.17803679510696002No Hit
ACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGGAACTC70850.17768568718591515RNA PCR Primer, Index 1 (100% over 26bp)
TAGTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACTTG67990.17051305394171307No Hit
TGACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCA67590.16950988845301349No Hit
CTGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCT64160.16090774438741448No Hit
TTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAATTGCTC64050.1606318738780221No Hit
GTCAAGTAATTAAGTGCATGTGGTGGAATTCTCGGGTGCCAAGGAACTCC61500.1542366938875622RNA PCR Primer, Index 1 (100% over 27bp)
GTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAA58720.14726469374110004No Hit
AATACTAATTGCTCGTGAGGCTTGACGTGGAATTCTCGGGTGCCAAGGAA55330.138762866224371RNA PCR Primer, Index 1 (100% over 23bp)
CACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCAC54670.13710764316801669No Hit
AACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTC54620.13698224748192922No Hit
AGTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACTTGG52810.13244292364556357No Hit
AAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGT49920.12519505298970904No Hit
AGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTG49700.12464331197092424No Hit
ACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACC48930.12271221840517753No Hit
GAATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCA47170.11829829025489934RNA PCR Primer, Index 1 (100% over 28bp)
TCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAAGGAACTCCA46680.11706941253124233RNA PCR Primer, Index 1 (100% over 28bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC46360.11626688014028266RNA PCR Primer, Index 1 (100% over 33bp)
GCTGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTC46190.11584053480758534No Hit
TTCGAATCTCTCTGAGCGCACCGTGGAATTCTCGGGTGCCAAGGAACTCC45860.11501292327940817RNA PCR Primer, Index 1 (100% over 27bp)
GACCAATACTAATTGCTCGTGAGGCTTGACGTGGAATTCTCGGGTGCCAA44710.11212882249939686No Hit
TGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGGGTGCCAAGGAACTCC44570.11177771457835199RNA PCR Primer, Index 1 (100% over 27bp)
TAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGGAACTCCA44130.11067423254078243RNA PCR Primer, Index 1 (100% over 28bp)
CAGTAATGCATTAAGCTAACCAGTACTAATTGCCCGTGCGGCTTGACTGG43900.11009741238478019No Hit
GTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAAGGAACTC43850.10997201669869273RNA PCR Primer, Index 1 (100% over 26bp)
TAGAGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAG43820.10989677928704025No Hit
ATAGAGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGA43510.10911932603329808No Hit
CGGGAGTTCGAATCTCTCTGAGCGCACCGTGGAATTCTCGGGTGCCAAGG43280.10854250587729582Illumina Small RNA Adapter 2 (100% over 21bp)
ACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCG43260.10849234760286082No Hit
TATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGGGTGCCAA43040.10794060658407606No Hit
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACG42840.10743902383972626RNA PCR Primer, Index 1 (100% over 39bp)
CGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTC41910.10510666407849971No Hit
ATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGGGTGCCAAG41910.10510666407849971No Hit
AGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGC41740.10468031874580237No Hit
TTGTAATCAGTAGGTCCACAGTTCGAATCCGTGTGCCGGCACCATGGAAT41500.10407841945258263No Hit
AGAGCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGC40580.10177113882857357No Hit
GCACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCA40200.10081813161430894No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)