FastQCFastQC Report
Wed 8 Jan 2025
SRR2125770.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2125770.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2840757
Total Bases143.8 Mbp
Sequences flagged as poor quality0
Sequence length20-51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC2315228.150010718973851RNA PCR Primer, Index 6 (100% over 50bp)
CCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC980293.450805542325514RNA PCR Primer, Index 6 (100% over 36bp)
CACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCA509811.7946272771659104RNA PCR Primer, Index 6 (100% over 37bp)
ACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC322791.1362816319734494RNA PCR Primer, Index 6 (100% over 35bp)
TGTAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC244610.8610732984201043RNA PCR Primer, Index 6 (98% over 50bp)
CACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG221130.7784192734542237RNA PCR Primer, Index 6 (100% over 34bp)
GTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGG159930.5629837398974992No Hit
CGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAAT149130.5249657045639595RNA PCR Primer, Index 6 (100% over 39bp)
TCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAAG147250.5183477502651582No Hit
GACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAA112040.3944019147009054No Hit
TCGGGAGTTCGAATCTCTCTGAGCGCACCGTGGAATTCTCGGGTGCCAAG97170.34205671234815227No Hit
AATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGGAA96140.33843091823763877RNA PCR Primer, Index 1 (100% over 23bp)
CCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCT91740.3229420890276782RNA PCR Primer, Index 6 (100% over 43bp)
ACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAG79360.27936215593238No Hit
TTGATAGGTTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACT76930.2708080979823336No Hit
ATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCAAGGAAC69960.2462723844383733RNA PCR Primer, Index 1 (100% over 24bp)
CGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAAGG59350.20892318491162742Illumina Small RNA Adapter 2 (100% over 21bp)
GTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATG56320.19825701388749548RNA PCR Primer, Index 6 (100% over 49bp)
TGACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGCCA52910.18625317124977603No Hit
GACCAATACTAATTGCTCGTGAGGCTTGACGTGGAATTCTCGGGTGCCAA51620.18171212814049215No Hit
TTGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC49970.1759038171867569RNA PCR Primer, Index 6 (98% over 50bp)
TGCTGGCGACAATAGCAAGAGTGAACCACCTGATCCCTTCCCGAACTC48090.16928586288795555No Hit
ATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGGGTGCCAAG46470.16358315758792463No Hit
CTGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCT45880.1615062463984072No Hit
ACGTTCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATAT45840.16136543886013482RNA PCR Primer, Index 6 (100% over 41bp)
GTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCTCGGGTGCCAA45740.1610134200144539No Hit
GTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGT45400.15981655593913874RNA PCR Primer, Index 6 (100% over 46bp)
ACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAA45220.1591829220169131RNA PCR Primer, Index 6 (100% over 38bp)
AATACTAATTGCTCGTGAGGCTTGACGTGGAATTCTCGGGTGCCAAGGAA45120.1588309031712322RNA PCR Primer, Index 1 (100% over 23bp)
TAGTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACTTG42360.14911518303043872No Hit
TTGGATGTGGAAGCATAGTGATATGTGAAGCTGACCAATACTAATTGCTC41300.14538378326622095No Hit
TATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGGGTGCCAA41270.14527817761251668No Hit
TGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCTG39850.14027951000384756No Hit
AGTGATATGTGAAGCTGACCAATACTAATTGCTCGTGAGGCTTGACTTGG37550.13218307655318637No Hit
CGGGAGTTCGAATCTCTCTGAGCGCACCGTGGAATTCTCGGGTGCCAAGG37450.13183105770750542Illumina Small RNA Adapter 2 (100% over 21bp)
CGTTCCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATC37240.13109181813157547RNA PCR Primer, Index 6 (100% over 42bp)
TTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATAT34480.12137609799078204RNA PCR Primer, Index 6 (100% over 41bp)
CCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC34360.12095367537596494RNA PCR Primer, Index 1 (100% over 33bp)
ACCAATACTAATTGCTCGTGAGGCTTGACGTGGAATTCTCGGGTGCCAAG34210.12042564710744355No Hit
TGGAATCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGCC33500.11792631330310899RNA PCR Primer, Index 6 (97% over 47bp)
ATACTAATTGCTCGTGAGGCTTGACGTGGAATTCTCGGGTGCCAAGGAAC32700.11511016253766163RNA PCR Primer, Index 1 (100% over 24bp)
GCTGATGGTAGTGTGGAGTTATCCATGTGAGAGTAAGTCATCGCCAGCTC32190.11331486642468891No Hit
AATTCCACCACGTTCCCGTGGTGGAATTCTCGGGTGCCAAGGAACTCCAG31990.11261082873332706RNA PCR Primer, Index 1 (100% over 29bp)
CAGTAATGCATTAAGCTAACCAGTACTAATTGCCCGTGCGGCTTGACTGG30720.10814018939317935No Hit
GGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGCC30250.10648570081847901RNA PCR Primer, Index 6 (100% over 50bp)
GAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAATTCT29960.10546484616600434No Hit
GCTGACCAATACTAATTGCTCGTGAGGCTTGACTTGGAATTCTCGGGTGC29890.1052184329740277No Hit
AGTTATCCATGTGAGAGTAAGTCATCGCCAGCTCTGGAATTCTCGGGTGC29770.1047960103592106No Hit
TGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCACCATGGAAT29430.10359914628389544No Hit
GTCGGGAGTTCGAATCTCTCTGAGCGCACCGTGGAATTCTCGGGTGCCAA29150.10261349351598888No Hit
CACTTGGCTGCGAACTAAGGGGTCGGGAGTTCGAATCTCTCTGAGCGCAC28760.10124062001783327No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)