FastQCFastQC Report
Wed 8 Jan 2025
SRR2125771.clean.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2125771.clean.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12749515
Total Bases533.2 Mbp
Sequences flagged as poor quality0
Sequence length20-75
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGTTCTACAGTCCGACGATC1142000.8957203470092785Illumina DpnII expression Sequencing Primer (100% over 22bp)
ACGTCCCGAGGCGCTGAACCTTGATGCTTGGAATTTCATGCTCAGGGACT494830.38811672444010614No Hit
CATCCCCTTTAATAGCGAGTGGTCTTTGTGTGCATCTCCGCAAACCACTC357130.28011261604853205No Hit
AGTTCTACAGTCCGACGATC354380.27795567125494575No Hit
GGCATCGCCGTCTCGACTTTTACTAGGCGGCGCAGCCGATTAGCGTTTAA325190.2550606827004792No Hit
CAGAGTTCTACAGTCCGACGATC324370.25441752098020987Illumina DpnII expression Sequencing Primer (100% over 23bp)
ATCTCGAATCGCCACTTCTCTTATCTGGTGGGCTCTGCCCGCCCTTGTCG278700.21859655053545177No Hit
AAGTTCTACAGTCCGACGATC277350.21753768672769122Illumina DpnII expression Sequencing Primer (95% over 21bp)
GCGCGGTTAGTCTAGTGGAAAGTCACTCGCAC273090.21419638315653575No Hit
GGTAAGACGGAGGTCGGGGGTTCGATCCCCCCAGTGGCCTCCA269430.21132568572216273No Hit
TACGTCCCGAGGCGCTGAACCTTGATGCTTGGAATTTCATGCTCAGGGAC267770.21002367541039796No Hit
TTCAGAGTTCTACAGTCCGACGAT240270.1884542274745353Illumina DpnII expression Sequencing Primer (100% over 24bp)
CTGAACCTTGATGCTTGGAATTTCATGCTCAGGGACTA211040.16552786517761656No Hit
TTCTCGGCTGAACGCCTCTAAGCCAGAAACCAGTCCCAAGACCGGGTGCC200820.1575118739810887No Hit
TCTCGAATCGCCACTTCTCTTATCTGGTGGGCTCTGCCCGCCCTTGTCGG194670.15268816107906852No Hit
ATATATATAACTAACGAATTACTATTTTGATATCTTGGACGTCGAATTAA192360.15087632745245605No Hit
TGAACCTTGATGCTTGGAATTTCATGCTCAGGGACTA191040.1498409939515346No Hit
ATCTGGTGGGCTCTGCCCGCCCTTGTCGGCGCCCAGTACCTTCATTTTCA189780.14885272106429148No Hit
ATCTCGAATCGCCACTTCTCTTATCTGGTGGGCTCTGCCCGCCC185080.1451663063261622No Hit
GAGTTCTACAGTCCGACGATC177570.13927588618076844Illumina DpnII expression Sequencing Primer (100% over 21bp)
ATATATATAACTAACGAATTACTATTTTGATATCTTGGACGTCGAA174340.1367424564777562No Hit
TCAGAGTTCTACAGTCCGACGAT154160.12091440341063953Illumina DpnII expression Sequencing Primer (100% over 23bp)
ATCTCGAATCGCCACTTCTCTTATCTGGTGGGCTC151270.1186476505184707No Hit
ATATAAAACCACAAAGGTAGAAGATCGTAATAGAGATAGAATAATATTTT139770.10962769956347358No Hit
AAATATAAAACATCAAGATAAATGGATTGTGATAGAGAAAGTTAAATTTT139550.1094551439799867No Hit
AACCTTGATGCTTGGAATTTCATGCTCAGGGACTA137570.10790214372860459No Hit
TCTCGGCTGAACGCCTCTAAGCCAGAAACCAGTCCCAAGACCGGGTGCCC136120.10676484556471363No Hit
ATACATATAAAAACGCAGAGATTGGTTATCACGGATTGGTAGATTAATTT134560.10554126960907925No Hit
ATATAAAATCTAATGACGAGAACTTGTATATGATGATGAAGGTGATAATT130540.10238820849263679No Hit
ATGTCTGTCAACTTGTTTTTT129220.10135287499171536No Hit
CGCTGAACCTTGATGCTTGGAATTTCATGCTCAGGGACTA128570.10084305167686772No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)